Instech Blog

Guide to Vascular Access Buttons™

Everything you need to know about VABs™.

The Vascular Access Button™ System

Our Vascular Access Buttons™ for mice and rats are built around our PinPort™ technology that simply and cleanly connects an implanted catheter to a syringe for direct access, or to a tether for continuous access. The VAB™ is a closed system as opposed to the traditional externalized or "open" systems. The PinPorts™ ensure all the ports remain closed - the magnetic cap simply allows for group housing after infusion or the samples have been taken.

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What are the advantages of using a VAB™?

Low-stress Sampling and Infusion

No poking. No needles. Every time you pick up an animal, stress levels rise. Every time you poke an animal, it gets worse. There is a better way! Hands off rodent blood sampling allows you to take precise, low stress blood samples all without using a needle. Take your samples from outside the cage using a swivel and tether. For even faster collection, use a capillary tube with injector. Hands free sampling - better for the animal, and better for you.

Proven Long-term Patency

At the American College of Toxicology’s 2019 meeting, Charles River Laboratories presented a poster on the extraordinary patency they achieved during a large infusion toxicology study in rats: flushing only every other week and locking with heparinized saline, CRL kept 94% of the rats’ femoral vein catheters patent for both sampling and infusion throughout the five-month study. This chart shows the impact of transcutaneous buttons on jugular vein patency in mice with flushing every seven days.

Patency of jugular vein catheters in mice

A separate study in mice found that the use of transcutaneous buttons kept catheters patent for up to five times longer than standard externalized catheters.

Another way to extend patency is to always ensure you are using pharmaceutical-grade lock and flush solutions. Non-sterile solutions can lead to patency problems, chemical purity issues, and more.

Learn Why

Group Housing

Rats being group-housed using the VAB with protective aluminum capFor the first time, rodents with externalized catheters can be group housed. Thanks to protective aluminum caps, you no longer have to trade off this basic animal welfare requirement with the benefits of sampling and infusing through a catheter.

What to Do When You Need Catheterized Rodents But You Don’t Have a Surgeon On Staff

To do the VAB™ surgeries in house you’ll need a trained surgeon with a steady hand, a dedicated surgical area with specialized surgical tools and microscopes, and SOPs to ensure aseptic technique. For some, this is a viable option. For others it's simply not possible. What do you if you are one of those researchers? Fortunately, there are a number of options available from in person training courses to animal-vendors that provide mice and rats that are already surgically modified.

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Does Instech offer any surgery training courses or advice?

We have been asked this question often throughout the years. Answers to some common questions such as "What Does it Take to Disconnect a Rodent Catheter?" can be found in our blog. There are some essential tips, such as always using a positive pressure technique, and focusing on sterility which helps improve patency. But now we have on our staff someone experienced with both surgery and educational delivery. 

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Are there any video resources available?

Use of the Vascular Access Button in Rats

Use of the Vascular Access Button in Mice

Rat Blood Sampling Outside the Cage

Refining Rodent Vascular Access: A Video Poster Presentation

IV Self-Administration in Rats

Automated Blood Sampling from a Mouse

Is the VAB™ only for mice and rats?

The VAB is most often used for mice and rats, but there have been a growing number of successful studies done on other models including ferrrets, hamsters, rabbits and minipigs.

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Have there been any publications demonstrating VABs™ in use?

Rats

Ferring Fewer complications compared to harnesses
AstraZeneca Fewer complications compared to harnesses
Charles River 94% bi-directional patency after 151 days
Charles River Comparison of JVC and FVC patency in rats
Charles River Tethered male and female rat study
Charles River Minimizing handling with the VAB™ in female rats

 

Mice

Charles River 100% patency in 28-day continuous infusion study
Charles River Patency 5x longer than externalized catheters
Charles River 84% patency for bolus dosing after 9 weeks
Charles River Use in reproduction studies
Charles River Comparison of patency with and without maintenance
Charles River Refinements to intermittent IV infusion in mice
The Jackson Laboratory Refinement of jugular vein catheterization with Vascular Access Button™ in mice
The Jackson Laboratory Self administration in mice

 

Other Species

Ellegaard Implantation in minipigs
Frontage Blood sampling from minipigs
US Army Blood sampling from ferrets
Charles River

JVC patency in hamsters

Charles River

Improving animal welfare in rabbits

 

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Rat Vascular Access Buttons

VAB™ for Rats

Vascular Access Buttons for Mice

VAB™ for Mice