Our pumps, swivels and tethers are standard equipment for mouse glucose clamp experiments. Both the infusion and sampling lines are run through a 375/D/25LT low-torque two-channel swivel connected to a two-channel mouse Vascular Access Button™. The infusion line is connected to a jugular vein catheter, the sampling line to a carotid artery catheter. While the surgery to place two catheters is difficult, collecting blood outside the cage, rather than from the tail or other acute methods, minimizes animal stress which will impact the data.
The Vascular Access Button™ permits quick, aseptic connection and disconnection of the IV lines, and also allows the mice to be group housed when they are not on study. The VABM2T/25GCY tether uses a looped wire to connect to the swivel so that the lines are visible, and includes a Y connector built-in to the button connector so that tracer injections can be made close to the animal. The HA1100WGC pump has user interface software designed specifically for glucose clamp studies.
This configuration is based on the clamp technique used and taught by the Vanderbilt MMPC, but using the a VAB™ in place of a researcher-constructed MASA device. Other configurations are possible.
A typical application setup is listed below. Add all the pieces to your quote, build your quote piece by piece, or call one of our experienced team members to help guide you through the system best suited for your needs.
This 90 second video will give you an overview of the surgery and equipment required for glucose clamp experiments in freely-moving mice.